But thank God it was just a scare.
For the past 3 weeks of micro lab, our aim was to do a transposon mutagenesis. Boring stuff, won't explain all that but anyway should see 3 bands and 5 bands alternatively besides the marker DNA on the first lane of each gel photo.
The results of 13 hours of HARD work.Anyway, took the 1st photo and got the scare of my life when I saw that there wasn't any bands at all for the first few lanes. The lab demonstrators said it could be contamination- meaning I would have to repeat 1 week's worth of experiments all over again. Which means extra time at lab, less time to write report, extra brain cells dying due to stress.
But I still ran the 2nd gel anyway hoping for a miracle. And guess what? It worked! (see 2nd gel). The bands were perfect!!! Then I realised I had made a serious, crazy mistake: throwing out the 1st gel into the rubbish bin, as I could have just stained it longer to get clearer results. So one little mistake costed me more than 3 hours of extra lab work. But yeah, final results were good, which meant I don't have to go into micro lab tomorrow! =D
*crosses fingers and hopes all goes well for bioc lab too!*
We had a 3rd year pHD student from micro department come in to talk about her research and life (or lack of) as a post grad student today. Very insightful. She has the brains, and got a full scholarship including a 25K funding for her research (a potential lead on TB disease? hmm), 10K for travel to a conference of her choice, and full tuition fees paid. Very very nice package, eh? Downside is, she stays at the lab from 8am to 7pm every day (sometimes weekends too). And though it's been 3 years doing same research, but still not sure where it'll lead her etc. It got me thinking quite a bit: on whether I would consider doing postgrad or not and where it'll lead me- industry or academics. The latter is just not for me: facing students and lecturing whole day. Oh well, time will tell.
A certain miss from IMB still isn't replying me!!! So frustrated since Ron got a reply from her ages ago, yet I sent an email to her first, TWICE!!! Discrimination! Very annoying since I'd even gotten a letter of endorsement for IMB, and really hoping to get accepted there for work placement end of this year.
If only accommodation in Singapore isn't that expensive...
Anyway, had a very good bday dinner (Ben's) at blue blue de sky tonight. It was almost like everyone sniffed some laughing gas: we couldn't stop laughing at the slightest things and talking about very random things, some quite gross. Like when some rice fell into a glass, the convo turned into drinking barley. Felt like we were kids once again. Ahhh, what a warm and fuzzy feeling that is. :P
3 weeks to finals. Eeks.
But I still ran the 2nd gel anyway hoping for a miracle. And guess what? It worked! (see 2nd gel). The bands were perfect!!! Then I realised I had made a serious, crazy mistake: throwing out the 1st gel into the rubbish bin, as I could have just stained it longer to get clearer results. So one little mistake costed me more than 3 hours of extra lab work. But yeah, final results were good, which meant I don't have to go into micro lab tomorrow! =D
*crosses fingers and hopes all goes well for bioc lab too!*
We had a 3rd year pHD student from micro department come in to talk about her research and life (or lack of) as a post grad student today. Very insightful. She has the brains, and got a full scholarship including a 25K funding for her research (a potential lead on TB disease? hmm), 10K for travel to a conference of her choice, and full tuition fees paid. Very very nice package, eh? Downside is, she stays at the lab from 8am to 7pm every day (sometimes weekends too). And though it's been 3 years doing same research, but still not sure where it'll lead her etc. It got me thinking quite a bit: on whether I would consider doing postgrad or not and where it'll lead me- industry or academics. The latter is just not for me: facing students and lecturing whole day. Oh well, time will tell.
A certain miss from IMB still isn't replying me!!! So frustrated since Ron got a reply from her ages ago, yet I sent an email to her first, TWICE!!! Discrimination! Very annoying since I'd even gotten a letter of endorsement for IMB, and really hoping to get accepted there for work placement end of this year.
If only accommodation in Singapore isn't that expensive...
Anyway, had a very good bday dinner (Ben's) at blue blue de sky tonight. It was almost like everyone sniffed some laughing gas: we couldn't stop laughing at the slightest things and talking about very random things, some quite gross. Like when some rice fell into a glass, the convo turned into drinking barley. Felt like we were kids once again. Ahhh, what a warm and fuzzy feeling that is. :P
3 weeks to finals. Eeks.
1 comments:
hey lucky i am not doing microb! i hate running gel and bacterial stuff! n biochem! argh! anyway, glad that ur results were good!
cant wait for u to come here! and we can really take nice nice pics since we never take any nice ones everytime we meet up...
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